I joined the Mycobacteria/Nocardia Laboratory as a volunteer in September 2019, contributing
to data entry, Quality Management activities, and projects involving Omadacycline
and Eravacycline. Currently, I serve as the Clinical Reference Laboratory Supervisor,
assisting in the oversight of both the molecular diagnostics and antimicrobial susceptibility
testing sections. My work includes sequencing of partial 16S rRNA, rpoB, erm(41),
and erm(39) gene regions for the identification of nontuberculous mycobacteria (NTM)
and other aerobic actinomycetes. I am also involved in studying inducible macrolide
resistance mechanisms, specifically erm(55), in NTM. In addition to diagnostic and
resistance testing, I have played an active role in developing protocols for DNA extraction,
library preparation, and downstream analysis for whole genome sequencing (WGS). Over
the past five years, I have worked extensively in the Mycobacteria/Nocardia laboratory,
with a focus on the identification and characterization of NTM, particularly various
subspecies of Mycobacterium abscessus. This work has contributed to understanding
patterns of macrolide resistance and supported several smaller research projects involving
NTM.